mouse monoclonal anti col1 e3e1x  (Cell Signaling Technology Inc)

Journal: Cell reports

Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

doi: 10.1016/j.celrep.2024.114527

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-COL1 (E3E1X) , Cell Signaling Technology , Cat# 66948S; RRID: AB_2920541.

Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western

Journal: Cell reports

Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

doi: 10.1016/j.celrep.2024.114527

Figure Lengend Snippet:

Article Snippet: For DKK1-, SPARC-, TIMP2-, COL1, integrin β2, or integrin α3 PLA, purified rabbit monoclonal anti-DKK1 (1:200, Cell Signaling Technology, Cat# 48367S), rabbit polyclonal anti-SPARC (1:200, Cell Signaling Technology, Cat# 5420S), mouse monoclonal anti-TIMP2 (1:200, R&D Systems, Cat# MAB971), mouse monoclonal anti-COL1 (1:200, Cell Signaling Technology, Cat# 66948S), goat polyclonal anti-integrin β1 (1:200, R&D Systems, Cat# AF1778), goat polyclonal anti-integrin β2 (1:200, R&D Systems, Cat# AF1730), and mouse monoclonal anti-integrin α3 (1:200, R&D Systems, Cat# MAB1345) were used.

Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western

Journal: Tissue Engineering and Regenerative Medicine

Article Title: In Vitro Assessment of Injectable Bone Marrow Aspirate Concentrates Compared to Injectable Platelet-Rich Fibrin

doi: 10.1007/s13770-024-00677-7

Figure Lengend Snippet: List of primer sequences for real-time PCR

Article Snippet: The RGFs were fixed with 4% formaldehyde for 10 min, permeabilized with PBS containing 0.2% Triton X-100, and blocked with PBS containing 1% bovine serum albumin (BSA; Sigma Aldrich) for 1 h. Subsequently, the cells were incubated overnight at 4 °C with an anti-mouse monoclonal COL1 antibody (MA1-26771; Invitrogen, Waltham, MA, USA) diluted 1:200 in PBS containing 1% BSA.

Techniques: Sequencing

Journal: Tissue Engineering and Regenerative Medicine

Article Title: In Vitro Assessment of Injectable Bone Marrow Aspirate Concentrates Compared to Injectable Platelet-Rich Fibrin

doi: 10.1007/s13770-024-00677-7

Figure Lengend Snippet: A Real-time PCR was performed to determine the relative mRNA levels of transforming growth factor-beta (TGF-β), vascular endothelial growth factor (VEGF) and collagen I (COL1) in RGFs cultured with control, iPRF, or iBMAC at 3 and 7 days postseeding. B Representative immunofluorescence staining of COL1 (green) with DAPI staining (blue) of RGFs at 14 days and C the quantified values of COL1 staining in comparison with each group. (# denotes significantly lower than all other modalities, p < 0.05; and ** denotes values significantly greater than those of all other treatment modalities, p < 0.05)

Article Snippet: The RGFs were fixed with 4% formaldehyde for 10 min, permeabilized with PBS containing 0.2% Triton X-100, and blocked with PBS containing 1% bovine serum albumin (BSA; Sigma Aldrich) for 1 h. Subsequently, the cells were incubated overnight at 4 °C with an anti-mouse monoclonal COL1 antibody (MA1-26771; Invitrogen, Waltham, MA, USA) diluted 1:200 in PBS containing 1% BSA.

Techniques: Real-time Polymerase Chain Reaction, Cell Culture, Control, Immunofluorescence, Staining, Comparison

Journal: Tissue Engineering and Regenerative Medicine

Article Title: In Vitro Assessment of Injectable Bone Marrow Aspirate Concentrates Compared to Injectable Platelet-Rich Fibrin

doi: 10.1007/s13770-024-00677-7

Figure Lengend Snippet: A Real-time PCR was performed to determine the relative mRNA levels of runt-related transcription factor 2 (Runx2), COL1 and osteocalcin (OCN) after 3 and 14 days of culture with control, iPRF or iBMAC in ROBs. B Representative alizarin red staining of ROBs 14 days after cell seeding and C the quantified values. (* denotes a statistically significant difference between groups, p < 0.05; # denotes a significantly lower value than that of all the other modalities, p < 0.05; and ** denotes a significantly greater value than that of all other treatment modalities, p < 0.05)

Article Snippet: The RGFs were fixed with 4% formaldehyde for 10 min, permeabilized with PBS containing 0.2% Triton X-100, and blocked with PBS containing 1% bovine serum albumin (BSA; Sigma Aldrich) for 1 h. Subsequently, the cells were incubated overnight at 4 °C with an anti-mouse monoclonal COL1 antibody (MA1-26771; Invitrogen, Waltham, MA, USA) diluted 1:200 in PBS containing 1% BSA.

Techniques: Real-time Polymerase Chain Reaction, Control, Staining